[Home ] [Archive]    
:: Volume 16, Issue 1 (Spring 2014) ::
J Mil Med 2014, 16(1): 45-51 Back to browse issues page
Study of the effect of sulfur mustard on cells viability and DNA fragmentation in the human skin fibroblast cells
Mahvash Jafari , Gilla Pirzad * , Ali Zaree , Mehdi Saberi
Researcher of Physiology, Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran , pirzad@razi.tums.ac.ir
Abstract:   (5362 Views)

Aims: Sulfur mustard (SM) is an alkylating and blistering agent that has readily reacts with a wide range of cellular macromolecules including DNA, RNA and protein. In this study, the effect of SM on cells viability and DNA fragmentation in the human fibroblastic cells was investigated.

Methods: the HF2FF human skin fibroblast cell line were exposed to various concentrations of SM (180-1000 mM) and then incubated for 24 hours at standard condition. Then, the effect of SM was investigated by measuring percent of viable cells using gentian violet dye assays and DNA fragmentation by agarose gels electrophoresis and diphenylamine reaction.

Results: Viability of the cells exposure to 180, 300 and 300 mM SM was 65, 42 and 16%, respectively. DNA fragmentation was increased and represented a smear pattern on agarose gel electrophoresis after exposure to higher concentrations of SM (>180 mM).

Conclusion: The effect of SM on viability and DNA damage is dose- dependent. At higher of SM concentrations (>180 µM), SM alkylates DNA, leading to DNA strand breaks and the nature of the DNA fragments produced suggested that necrotic form of cell death.

Keywords: Sulfur Mustard, HF2FF Cell line, Viability, DNA Fragmentation
Full-Text [PDF 783 kb]   (1809 Downloads)    
Type of Study: Research | Subject: military medcine
Received: 2012/07/1 | Accepted: 2014/06/22 | Published: 2014/06/22
Send email to the article author


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jafari M, Pirzad G, Zaree A, Saberi M. Study of the effect of sulfur mustard on cells viability and DNA fragmentation in the human skin fibroblast cells. J Mil Med. 2014; 16 (1) :45-51
URL: http://militarymedj.ir/article-1-1021-en.html


Volume 16, Issue 1 (Spring 2014) Back to browse issues page
مجله طب‌نظامی Journal Mil Med
Persian site map - English site map - Created in 0.07 seconds with 30 queries by YEKTAWEB 3781