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:: Volume 18, Issue 1 (Spring 2016) ::
J Mil Med 2016, 18(1): 315-322 Back to browse issues page
Evaluation of the antibacterial effects of Arctium lappa extracts on Brucella melitensis 16M in the animal model and macrophage culture
Sh Nassiri-Semnani *, F Gholikhani, M Rahnema, R Shapouri, H Alizadeh, H Gassempour
Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran , Sh.nasiri92@yahoo.com
Abstract:   (3277 Views)

Aims: The aim of this study was to evaluate the antibacterial effects of aqueous and organic extracts of Arctium lappa on Brucella melitensis 16M in vitro, in macrophage culture and in the animal model.
Methods: In this experimental study aquatic, ethanolic, and acetonic extracts of Arctium lappa were prepared. The antibacterial effect of the extracts investigated by agar well diffusion and macrodilutionon methods for determination of MIC and MBC. Then the intramacrophge survival of the B.melitensis 16M was studied from the cell culture of Balb/c mice. Also, the antibacterial effect of the extracts were studied in the animal model.
Results: The MIC and MBC of leaves and inflorescences extracts of Arctium lappa on B. melitensis 16 M were 101 and 116 mg/ml, respectively. The maximum inhibition zone diameter was related to an ethanolic extract. In the macrophage culture and in the animal model, the aquatic extract of inflorescences of Arctium lappa was the most effective.
Conclusion: The aquatic and organic extracts of Arctium lappa have antibacterial activity against intramacrophage B. Melitensis 16M. The aquatic extract has the most effective antimicrobial activity on intramacrophage B. Melitensis 16M. These extracts can be useful in the treatment of brucellosis.

Keywords: Antimicrobial, Brucella melitensis, Arctium lappa, Macrophage
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Type of Study: Orginal Research | Subject: military medcine
Received: 2016/12/3 | Accepted: 2016/12/3 | Published: 2016/12/3
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Nassiri-Semnani S, Gholikhani F, Rahnema M, Shapouri R, Alizadeh H, Gassempour H. Evaluation of the antibacterial effects of Arctium lappa extracts on Brucella melitensis 16M in the animal model and macrophage culture. J Mil Med. 2016; 18 (1) :315-322
URL: http://militarymedj.ir/article-1-1546-en.html


Volume 18, Issue 1 (Spring 2016) Back to browse issues page
مجله طب‌نظامی Journal Mil Med
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