Interoduction. T-2 toxin, an important member of trichothecene mycotoxins family, disturbs structures and functions of many parts of human and animal immune system. Our previous studies showed that peritoneal inoculation of T-2 toxin changes normal proportion of mouse peripheral blood lymphocytes and antioxidants may affect the phenomenon. In the present study, the effects of two well-known antioxidants, Selenium and Vitamin E, on these T-2 toxin induced abnormalities are compared.
Material and Methods. A single sub lethal dose of T-2 toxin (2 μg/Kg) inoculated to a group of mice and at different times after intoxication the peripheral blood lymphocytes counted by flow cytometry method. Then, 4 separate groups of mice received T-2 toxin and selenium sulphide or vitamin E, and proportion of the lymphocyte subpopulations were determined in their peripheral blood samples.
Results. The results showed that T-2 toxin affects normal proportion of all studied lymphocytes. CD19+ and CD8+ lymphocytes decreased and CD4+ cells increased after receiving a single sub lethal dose of the toxin. Peritoneal injection of selenium in both condition, concomitant or before T-2 toxin, prevented CD19+ cells reduction but, vitamin E had no effect on reduction process of this subpopulation. When, selenium inoculated at intoxication time, the increasing of CD4+ population prevented completely but, vitamin E had no efficacy in this condition. In contrast, injection of vitamin E , 24 h before the toxin, significantly affected the CD4+ proportion and prevented the increasing of the subpopulation while, selenium was ineffective in this manner. Receiving of vitamin E in both condition, before and at intoxication, prevented reduction of CD8+ cells while selenium repair the problem only when received concomitant with the toxin.
Discussion. It is concluded that to cure T-2 toxin induced abnormalities in lymphocyte subpopulations, efficacy of selenium and vitamin E varies depending on inoculation time of these antioxidants as well as type of disturbed lymphocyte, and it seems that selenium received immediately after intoxication provides more efficacious results while usefulness of vitamin E is more when received prior to intoxication.