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:: Volume 8, Issue 3 (fall 2006) ::
J Mil Med 2006, 8(3): 191-196 Back to browse issues page
Molecular Survey of Nalidixic Acid Resistance E.coli Isolated from Patients with Urinary Tract Infection in Baqiyatallah Hospital
Naghavi KH. A., Jonaidi N. *, Karami A., Sorouri R., Khalil pour A., Safiri Z., Majid far M., Dalili R.
, Jonaidi2000@yahoo.com
Abstract:   (28966 Views)

Introduction. Members of the Enterobacteriaceae family are a large group of bacteria widely distributed in nature. Enterobacteriaceae often are labeled as enteric bacteria because of their predilection for intestinal colonization. E.coli is the most common cause of urinary tract infection. The aim of this study was molecular survey of Nalidixic Acid Resistance E.coli isolated from patients with urinary tract infection in Baqiyatallah hospital.

Materials and Methods. During 5 months (January to June 2005), urine samples of patients referred to clinical laboratory of Baqiyatallah hospital were cultured. Differential tests were done for diagnosis of E.coli. For evaluation of antimicrobial resistance, PCR method was done for positive samples including sensitive and resistant E.coli. Then, MIC test was done and finally sequencing was performed.

Results. In this study, 121 E.coli were isolated from urinary samples. In Nalidixic Acid antibiogram test, 55 cases (45.5%) were sensitive, 63 cases (52%) were resistant and 3 cases (2.5%) were Intermediate. Results of PCR and MIC were similar to antibiogram.

Discussion. Depending on results of this study and comparison with other studies, trend of resistance of E.coli is increasing. Therefore, we recommend control of antibiotic misusage and application of MIC and PCR tests (if possible) prior to treatment for suitable selection of antibiotic and prevention of microbial resistance. 

Keywords: E.coli, Urinary tract infection, Antimicrobial resistance, Nalidixic Acid, PCR
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Type of Study: Orginal Research | Subject: military medcine
Received: 2007/05/10 | Published: 2006/10/15
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Naghavi KH. A., Jonaidi N., Karami A., Sorouri R., Khalil pour A., Safiri Z., et al . Molecular Survey of Nalidixic Acid Resistance E.coli Isolated from Patients with Urinary Tract Infection in Baqiyatallah Hospital. J Mil Med. 2006; 8 (3) :191-196
URL: http://militarymedj.ir/article-1-237-en.html


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Volume 8, Issue 3 (fall 2006) Back to browse issues page
مجله طب‌نظامی Journal Mil Med
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